[Home ] [Archive]   [ فارسی ]  
:: Main Online Submission Archive Current Issue In Press ::
:: Volume 14, Issue 8 (Clinical Science Issue - October 2012) ::
ZJRMS 2012, 14(8): 1-5 Back to browse issues page
Comparison of Traditional Methods and PCR for Diagnosis of Cutaneous Leishmaniasis in South-West of Iran
Shahram Khademvatan , Jasem Saki , Sharif Maraghi
, jasem.saki@gmail.com
Abstract:   (6140 Views)
Background: The usual method for laboratory diagnosis of cutaneous leishmaniasis was the direct observation of parasites under a light microscope. Although this method has high specificity, it has low sensitivity. The purpose of this study is to compare three methods of direct observation, culture and Mini-exon-PCR to diagnose cutaneous leishmaniasis in Khuzestan province. This study intends to compare sensitivity of PCR approach with sensitivity of the existing traditional methods to diagnose cutaneous leishmaniasis using Mini-exon gene.
Materials and Methods:
A total 216 skin biopsies prepared from patients with cutaneous leishmaniasis were studied though direct method, culture in NNN, culture in RPMI 1640 and Mini-exon-PCR and the sensitivity of these methods were compared with each other. In this study Mini-exon-PCR was considered as the gold standard method.
Results: Results showed that 46.7% with direct method, 35.1% with culture method in RPMI 1640, 57.8% with culture method in NNN and 70.3% with PCR were positive. Sensitivity was obtained 66.4% for microscopic observation, 50% for culture in RPMI1640, and 82.2% for culture in NNN and 100% for PCR.
Conclusion: This study showed that PCR on samples stored in normal saline has higher sensitivity and specificity than other traditional methods (p>0.05). Thus, Mini-exon-PCR on samples in normal saline is a reliable method to diagnose cutaneous leishmaniasis, especially in cases where the diagnosis is negative with the other methods.

Keywords: Traditional method, PCR, Cutaneous leishmaniasis, south-West of Iran
Full-Text [PDF 150 kb]   (1842 Downloads)    
Type of Study: Research | Subject: Parasitology
* Corresponding Author Address: Department of Medical Parasitology, Infectious and Tropical Diseases Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khademvatan S, Saki J, Maraghi S. Comparison of Traditional Methods and PCR for Diagnosis of Cutaneous Leishmaniasis in South-West of Iran. ZJRMS. 2012; 14 (8) :1-5
URL: http://zjrms.ir/article-1-1480-en.html

Volume 14, Issue 8 (Clinical Science Issue - October 2012) Back to browse issues page
Zahedan Journal of Research in Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 800 queries by yektaweb 3610