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ZJRMS 2009, 11(1): 0-0 Back to browse issues page
The role of serine/threonine protein phosphatases in the inhibitory effects of low frequency stimulasion in perforant path kindled seizures acquisition
M Sadegh , SJ Mirnajafizadeh
Abstract:   (8761 Views)

Introduction: The use of low-frequency electrical stimulation (LFS) as a therapy for epilepsy is currently being studied in experimental animals and patients with epilepsy. In the present study, the role of serine/threonine protein phosphatases in the inhibitory effects of LFS on perforant path kindling acquisition was investigated.

Methods: sixty four male Wistar rats were stimulated by perforant path stimulation in a rapid kindling manner (6 stimulations per day). LFS (1 Hz) was applied immediately after termination of each kindling stimulation. FK506 (1 µM i.c.v.), a serine/threonine protein phosphatase PP2B inhibitor and okadaic acid (1 µM i.c.v.), a serine/threonine protein phosphatases PP1/2A inhibitor, were daily microinjected into the left ventricle 10 min before starting the stimulation protocol.

A two-way ANOVA was done to compare the seizure parameters of different groups. The effect of LFS on behavioral seizure scores was analyzed by using the nonparametric Kruskal–Wallis and Mann–Whitney U test. P value less than 0.05 was considered as the level of significance.

Results: Application of LFS immediately after kindling stimulation significantly retarded the kindling acquisition and delayed the expression of different kindled seizure stages. In addition, LFS significantly reduced the increment of daily afterdischarge duration during kindling development. Microinjection of neither FK506 nor okadaic acid had significant effect on the antiepileptogenic effect of LFS on kindling parameters.

Conclusion: Obtained results showed that activation of PP1/2A and PP2B, which play a critical role in LFS induced down-regulation of synaptic strength, had no role in mediating the inhibitory effects of LFS on perforant path kindled seizures.

Keywords: Keywords : Seizure ; Epilepsy; Low-frequency stimulation; Kindling
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Type of Study: Research | Subject: Oncology
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Sadegh M, Mirnajafizadeh S. The role of serine/threonine protein phosphatases in the inhibitory effects of low frequency stimulasion in perforant path kindled seizures acquisition. ZJRMS. 2009; 11 (1)
URL: http://zjrms.ir/article-1-435-en.html


Volume 11, Issue 1 (Mar 2009) Back to browse issues page
Zahedan Journal of Research in Medical Sciences
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