:: Volume 17, Issue 2 (Basic Science Issue - February 2015) ::
ZJRMS 2015, 17(2): 27-30 Back to browse issues page
Morphometry of Rat Jejunal Enterocytes Number and Height after L-Arginine and L-NAME Administration
S. Mohammad Hossein Noori-Mugahi , Fatemeh Moghani-Ghoroghi
, noorioo@gmail.com
Abstract:   (11003 Views)
Background : Nowadays nitric oxide (NO) is an important signaling molecule which acts as a regulator of many physiological processes in many tissues including epithelial cell of gastrointestinal tract. In this study, we investigated the effects of L-Argentine as a NO precursor and L-NAME as a NO inhibitor on epithelial cell number and height of jejuna epithelium in rats.
Materials and Methods : In this experimental study, 40 adult female rats with 8 weeks age and 200-250 g weight were divided into 5 groups, containing 8 rats in each group. Except the control group, the other groups received normal saline (2 mL/kg), L-Argentine (200 mg/kg), L-NAME (20 mg/kg) and a mixture of 2 substances for L-Argentine and L-NAME group intraperitonealy for 3 days. Two weeks later, jejunum was expelled out and after fixation and tissue processing, the sections were stained with Hematoxylin & Eosin method and the changes were assessed. Cell number and height was evaluated using Image Tools III Microsoft software. Statistical analysis was made by One-Way ANOVA followed by Turkey post hoc test to evaluate the statistical significance between different groups. A value of p<0.05 was considered statistically significant.
Results : There was a significant increase in the cell number and height of jejuna epithelium in L-Argentine group (p<0.05). Whereas no significant difference was observed between L-NAME, L-Arginine+L-NAME, normal saline and control groups.
Conclusion : The results demonstrated that L-Argentine can result in proliferation of jejuna epithelial cells whereas L-NAME has no effect on these cells.
Keywords: Nitric oxide, L-Arginine, L-NAME, Epithelial cell, Jejunum
Full-Text [PDF 476 kb]   (1860 Downloads)    
Type of Study: Research | Subject: Histology
* Corresponding Author Address: Department of Histology, Tehran University of Medical Sciences, Tehran, Iran

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Volume 17, Issue 2 (Basic Science Issue - February 2015) Back to browse issues page