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:: Volume 16, Issue 12 (Basic Science Issue - December 2014) ::
ZJRMS 2014, 16(12): 25-28 Back to browse issues page
Microsatellite Instability Markers Status in Colorectal Cancer
Giti Esmailnia , Mahdi Montazer-Haghighi , Gholamreza Javadi , Kazem Parivar , Mohammadreza Zali
Department of Biology, Faculty of Science, East Tehran Branch, Islamic Azad University, Tehran, Iran , mah_haghighi@hotmail.com
Abstract:   (4178 Views)
Background : Colorectal cancer (CRC) is the third most prevalent and the third leading cause of cancer-related deaths in Iran. Our aim was to investigate five mononucleotide statuses among Iranian patients with sporadic colorectal cancer.
Materials and Methods : In this experimental study investigation 80 sporadic CRC patients were evaluated for Microsatellite instability (MSI). The pentaplex panel including 5 quasi mononucleotide microsatellite markers (NR-21, BAT-26, BAT-25, NR-27 and NR-24) was used. The MSI analysis was performed on paired tumoral DNA from cancerous tissues and genomic DNA from whole blood. MSI carriers were identified by analysis of tumor tissue using polymerase chain reaction.
Results : Our findings showed that microsatellite instability was detected in 36 of 80 cases (45%) with colorectal cancer. MSI analysis revealed that 17 cases of MSI-H (21%), 19 MSI-L (23%) and 44 MSS (55%). Instability is observed in the tumoral DNA compared to the DNA from the normal DNA sample. The most instable markers were NR-21, NR-24 in which instability was detected in 45% of patients.
Conclusion : Using a panel including 3 mentioned MSI markers should be more promising markers for identifying MSI status in patients with sporadic colorectal cancer.
Keywords: Sporadic Colorectal Cancer, MSI, Mononucleotide markers
Full-Text [PDF 206 kb]   (1404 Downloads)    
Type of Study: Research | Subject: Genetics and Molecular Biology
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Esmailnia G, Montazer-Haghighi M, Javadi G, Parivar K, Zali M. Microsatellite Instability Markers Status in Colorectal Cancer . ZJRMS. 2014; 16 (12) :25-28
URL: http://zjrms.ir/article-1-2870-en.html


Volume 16, Issue 12 (Basic Science Issue - December 2014) Back to browse issues page
Zahedan Journal of Research in Medical Sciences
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