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ZJRMS 2005, 7(1): 9-15 Back to browse issues page
Effects of estradiol, progesterone and testosterone on proliferation of human breast cancer cell lines
Abstract:   (7199 Views)

 
Hashemi M., PhD*, Ghavami S., PhD*
 
Background: Steroid hormones affect the proliferation of breast cancer. The aim of this study was to test the effect of estradiol, progesterone and testosterone on MCF-7 and MDA-MB468 human breast cancer cell line.
Methods and Materials: The human breast cancer cell line MCF-7 and MDA-MB468 were grown in charcoal striped serum in the presence and absence of 10 nM 17- β-estradiol with different concentration of progesterone and testosterone for 72 hrs. Growth of cells evaluated by MTT method.
Results: The results demonstrated that estradiol produced an increase in the cell proliferation rate of MCF-7 cells grown in the presence of charcoal-stripped serum, but had no effect on MDA-MB468 cells. While progesterone had no effect on cell growth when tested alone on both cell lines, it significantly inhibited the growth of MCF-7 cells in the presence of 10 nM estradiol. Testosterone had no effect on cell growth of MDA-MB468 cells in the presence or absence of estradiol. In MCF-7 cells, testosterone significantly inhibited the growth of these cells in the absence or presence of estradiol.
Conclusions: Proliferation of breast cancer cells are hormone dependent or hormone independent.
 
 
 
  *Clinical Biochemistry Dept, Faculty of Medicine, Zahedan University of Medical Sciences and health services, Zahedan, Iran.
 
 
 
 
 
 
 
 
 
Keywords: Breast cancer cell line, Proliferation, Steroid hormone
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Effects of estradiol, progesterone and testosterone on proliferation of human breast cancer cell lines. ZJRMS. 2005; 7 (1) :9-15
URL: http://zjrms.ir/article-1-18-en.html


Volume 7, Issue 1 (Mar 2005) Back to browse issues page
Zahedan Journal of Research in Medical Sciences
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