:: Volume 13, Issue 8 (January 2012) ::
ZJRMS 2012, 13(8): 1-7 Back to browse issues page
Evaluation of the efficacy of aminolevulinic acid-dependent photodynamic therapy on melanoma cancer cells treated with tocopherol succinate (in-vitro)
Homa Kouchesfahani, Kazem Parivar, Mohammad Nabiuni, Mohaddese Mohammadi-Sardoo
, shokouh_1980@yahoo.com
Abstract:   (7752 Views)

Background: Photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) to produce an intracellular  photo-sensitizer, a protoporphyrin molecule IX (PPIX) which absorbs light and targets cells, is a promising cancer treatment. Unfortunately, treatment failures are still a common occurrence when ALA is used. In this study, in order to enhance the efficacy of ALA-dependent photodynamic therapy, the effects of photodynamic therapy on melanoma cancer cells were studied after treating them with tocopherol succinate.

Materials and Methods: In this experimental study melanoma cells were cultured in RPMI 1640 medium for 24 h. then, cells were treated with tocopherol succinate (6μm/ml). After 48 and 72 hours, the mediums were replaced by serum-free medium in the darkness, with ALA, 0.1mg/ml and then cells incubated for 4h. After that, cells were irradiated by using Nd: YAG laser (532 nm). After 24h, cell survival was measured by the MTT assay.

Results: Twenty-four hours after PDT, among compared groups, pretreated cells with tocopherol succinate showed significant lower cell viability than control group.

Conclusion: Induction of differentiation by using tocopherol succinate augmented intracellular PPIX accumulation in cells treated with ALA. Therefore phototoxic cell death after exposure to 532nm light enhances significantly in tocopherol succinate-pretreated cells. This study suggests that tocopherol succinate may act as a biological enhancer of ALA based photodynamic therapy. [ZJRMS, 2011 13(8): 1-7]

 


Keywords: Keywords: Photodynamic therapy, aminolevulinic acid, tocopherol succinate, melanoma
Full-Text [PDF 265 kb]   (1780 Downloads)    
Type of Study: Research | Subject: Biology
* Corresponding Author Address: MSc of Biology, School of Science, Tarbiat Moallem University, Tehran, Iran.


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Volume 13, Issue 8 (January 2012) Back to browse issues page