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ZJRMS 2012, 14(8): 1-5 Back to browse issues page
Comparison of Traditional Methods and PCR for Diagnosis of Cutaneous Leishmaniasis in South-West of Iran
Shahram Khademvatan , Jasem Saki , Sharif Maraghi
, jasem.saki@gmail.com
Abstract:   (6324 Views)
Background: The usual method for laboratory diagnosis of cutaneous leishmaniasis was the direct observation of parasites under a light microscope. Although this method has high specificity, it has low sensitivity. The purpose of this study is to compare three methods of direct observation, culture and Mini-exon-PCR to diagnose cutaneous leishmaniasis in Khuzestan province. This study intends to compare sensitivity of PCR approach with sensitivity of the existing traditional methods to diagnose cutaneous leishmaniasis using Mini-exon gene.
Materials and Methods:
A total 216 skin biopsies prepared from patients with cutaneous leishmaniasis were studied though direct method, culture in NNN, culture in RPMI 1640 and Mini-exon-PCR and the sensitivity of these methods were compared with each other. In this study Mini-exon-PCR was considered as the gold standard method.
Results: Results showed that 46.7% with direct method, 35.1% with culture method in RPMI 1640, 57.8% with culture method in NNN and 70.3% with PCR were positive. Sensitivity was obtained 66.4% for microscopic observation, 50% for culture in RPMI1640, and 82.2% for culture in NNN and 100% for PCR.
Conclusion: This study showed that PCR on samples stored in normal saline has higher sensitivity and specificity than other traditional methods (p>0.05). Thus, Mini-exon-PCR on samples in normal saline is a reliable method to diagnose cutaneous leishmaniasis, especially in cases where the diagnosis is negative with the other methods.

Keywords: Traditional method, PCR, Cutaneous leishmaniasis, south-West of Iran
Full-Text [PDF 150 kb]   (1919 Downloads)    
Type of Study: Research | Subject: Parasitology
* Corresponding Author Address: Department of Medical Parasitology, Infectious and Tropical Diseases Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran
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Khademvatan S, Saki J, Maraghi S. Comparison of Traditional Methods and PCR for Diagnosis of Cutaneous Leishmaniasis in South-West of Iran. ZJRMS. 2012; 14 (8) :1-5
URL: http://zjrms.ir/article-1-1480-en.html

Volume 14, Issue 8 (Clinical Science Issue - October 2012) Back to browse issues page
Zahedan Journal of Research in Medical Sciences
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