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Comparison between C-FOS Expression in Male and Female Mice During Morphine Withdrawal in the Presence and Absence of Acute Administration of Matricaria Recutita
Mahnaz Kesmati , Hamid Galedari, Azar Massah
Associate
Abstract:   (13681 Views)
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Background: There are some evidences that indicate there are sexual differences in drug abuse and response to synthetic and herbal drugs. It has been shown that the expression of c-fos increases in many areas of brain during morphine withdrawal. Concerning the sedative effect of Matricaria recutita extract, the aim of this study was to compare expression of c-fos transcription factor during morphine withdrawal with and without acute administration of Matricaria recutita on male and female adult mice.

Materials and Methods: This study was done at Shahid Chamran University of Ahvaz in 2007 on NMRI mice. Male and female mice were assigned into 8 groups (morphine + saline morphine + naloxone morphine + Matricaria recutita + naloxone and morphine + saline + naloxone). To develop morphine dependency, increasing doses of morphine (20, 40, 80 mg/kg injected subcutaneously) for 4 days. Mice received a final morphine injection (40 mg/kg) 3hours prior to naloxone (5 mg/kg) on the day of testing (day 4). Matricaria recutita extract whit a dose of 30 mg/kg was administered intraperitoneally 5 minutes before naloxone injection. In cellular study, 90minute after naloxone injection, mice were decapitated and their brains were separated, then mRNA was extracted from brain tissue. Using DIG-labeled DNA probe of c-fos, beta-actin and dot blot technique, expression of c-fos was analyzed by Zero Dscan software. Statistical evaluation of data was performed using student t-test and ANOVA with one factor followed by Duncan test in SPSS software. P values less than 0.05 were considered significant.

Results: The rate of expression of c-fos increased in male mice but decreased significantly in female mice after naloxone-precipitated abstinence) P<0.01(. Matricaria recutita attenuated the rate of expression of c-fos in male mice but it showed synergistic effect on it in female mice )P<0.05(.

Conclusion: It seems that the cellular processes involving morphine dependency and response to treatment with Matricaria recutita are sex dependent and are affected by sexual hormones.

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Keywords: Morphine dependency, C-fos, Matricaria recutita, Mice
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Type of Study: Research | Subject: Epidemiology
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Kesmati M, Galedari H, Massah A. Comparison between C-FOS Expression in Male and Female Mice During Morphine Withdrawal in the Presence and Absence of Acute Administration of Matricaria Recutita. ZJRMS. 2009; 11 (2)
URL: http://zjrms.ir/article-1-471-en.html
Volume 11, Number 2 (Summer 2009) Back to browse issues page
Zahedan Journal of Research in Medical Sciences
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