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:: Volume 16, Issue 3 (Basic Science Issue - March 2014) ::
ZJRMS 2014, 16(3): 35-39 Back to browse issues page
In Vitro Antimicrobial Activity of Iris pseudacorus and Urtica dioica
Maryam Ramtin , Alireza Massiha , Mohammad Reza Majid Khoshkholgh-Pahlaviani , Khosro Issazadeh , Mehdi Assmar , Saied Zarrabi
, Mbio_Ramtin@yahoo.com
Abstract:   (13373 Views)
Background : In this study, the effects of antibacterial activity of Urtica dioica and Iris pseudacorus essential oils, native plant northern of Iran, were investigated for some selected bacteria.
Material and Methods : The influence of essential oils was tested by the using of disk diffusion and micro-broth dilution methods against standard strains of the picked out bacteria. Gas Chromatography/Mass Spectroscopy (GC/MS) analysis, bioactivity determination, Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) of essential oils were utilized for this goal.
Results : This study showed that, Inhibition zone diameter varied from 11 to 19 mm and 9 to 17 mm for Urtica dioica and Iris pseudacorus respectively. In contrast, this figure fluctuated from 19 to 28 mm and 7 to 17 mm for gentamicin and ampicillin separately. By the application of micro-broth dilution technique, MICs for 1% essential oils were 1.8-7.5 μg/ml and 3.75-15 μg/ml for, Urtica dioica and Iris pseudacorus against gram-positive and gram-negative bacteria individually. Furthermore, the MBCs of herbal essences were 1.8-15 μg/ml for, Urtica dioica and 15-30 μg/ml for Iris.
Conclusion : The application of essential oils for the bio-control of diseases, as a novel emerging alternative to antimicrobial treatments, lead to safer and more environmental management for infective diseases.
Keywords: Essentials oil, Iris Pseudacorus, Urtica dioica, Antimicrobial Activity
Full-Text [PDF 257 kb]   (2294 Downloads)    
Type of Study: Research | Subject: Microbiology
* Corresponding Author Address: Department of Microbiology, Member of Young Researchers Club of Lahijan, Lahijan Branch, Islamic Azad University, Lahijan, Iran
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Ramtin M, Massiha A, Khoshkholgh-Pahlaviani M R M, Issazadeh K, Assmar M, Zarrabi S. In Vitro Antimicrobial Activity of Iris pseudacorus and Urtica dioica . ZJRMS. 2014; 16 (3) :35-39
URL: http://zjrms.ir/article-1-2715-en.html


Volume 16, Issue 3 (Basic Science Issue - March 2014) Back to browse issues page
Zahedan Journal of Research in Medical Sciences
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