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:: Volume 16, Issue 4 (Clinical Science Issue - April 2014) ::
ZJRMS 2014, 16(4): 11-14 Back to browse issues page
Feasibility Study of Anti-Neurofilament Antibodies Detection by Indirect Quenching Fluoroimmunoassay
Alireza Nakhaee, Manoochehr Messripour , Alireza Khosravi, Saeedeh Salami, Gholamhosein Aliahmmad
Department of Biochemistry Isfahan Branch, Islamic Azad University Khorasgan, Isfahan, Iran , m_messripour@yahoo.com
Abstract:   (5867 Views)
Background : Neurofilaments (NFs) are the main constitutes of intermediate filaments in neurons. They are composed of three subunits with heavey, medium and low molecular weight. Anti-neurofilament antibodies exist in serum of patients with some neurodegenerative diseases.
Materials and Methods : A fluoroimmunoassay has been developed for determining of antibodies against neurofilaments, using an anti-fluorescein serum and fluorescein-labeled NFs. Antibodies raised against bovine spinal cord NFs in rabbit and the labeled NFs are incubated with anti-fluorescein serum at room temperature.
Results : At high levels, binding of anti-neurofilaments (anti-NFs) to labeled NFs prevented subsequent binding of the anti-fluorescein to fluorescein groups, resulting in little change in the signals of the label. Conversely, at low level of anti-NFs the free fraction of the labeled NFs is available to be bound by anti-fluorescein, which markedly reduced fluorescence intensity of label. Thus, the fluorescence intensity of assay mixture directly reflects the amount of anti-NFs antibodies in the serum.
Conclusion : It is concluded that the availability of fluorescein-labeled NFs and antibody directed against fluorescein group permit measurement of anti-NFs antibodies in serum of neurodegenerative patients.
Keywords: Neurofilament, Anti-neurofilament, Fluoroimmunoassay, Neurodegenerative disease
Full-Text [PDF 167 kb]   (1540 Downloads)    
Type of Study: Research | Subject: Immunology
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Nakhaee A, Messripour M, Khosravi A, Salami S, Aliahmmad G. Feasibility Study of Anti-Neurofilament Antibodies Detection by Indirect Quenching Fluoroimmunoassay. ZJRMS. 2014; 16 (4) :11-14
URL: http://zjrms.ir/article-1-2659-en.html


Volume 16, Issue 4 (Clinical Science Issue - April 2014) Back to browse issues page
Zahedan Journal of Research in Medical Sciences
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